Anti-Human IL17A/IL17F Recombinant Antibody (TAB-H08)

Name: Anti-Human IL17A/IL17F Recombinant Antibody (TAB-H08)
SKU: TAB-H08
Rating: 5 (1 reviews)

CAT#: TAB-H08

Recombinant Humanized antibody to Human IL17A

Tested Data Gene Expression

ELISA

Figure 1 Anti-Human IL17A Recombinant Antibody (TAB-H08) in ELISA

ELISA analysis of TAB-H08 was performed by coating with human IL17A protein (His tag). Then blocked with BSA and incubated with TAB-H08. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human IL17A Recombinant Antibody (TAB-H08) in WB

Western blot analysis of TAB-H08 was performed by loading human IL17A protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H08 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 3 Anti-Human IL17A Recombinant Antibody (TAB-H08) in DB

Dot Blot analysis of TAB-H08 was performed by coating with human IL17A protein (His tag).

TAB-H08 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti- IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

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Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows distinct positivity in peripheral leukocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_B_7_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_8_3.jpg

Normal Tissue Figure 4 Liver

Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_7_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_4_6.jpg

Normal Tissue Figure 7 Tonsil

Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_4_8.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (Karpas-707, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112115-IL17A

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Specifications

Host Species
Rat

Derivation
Humanized

Type
IgG1 - kappa

Specificity
IL17A (interleukin 17A, IL-17A) [Homo sapiens] ;

Species Reactivity
Human

Applications
ELISA, FC, IP, FuncS, IF, Neut, WB, Inhib, PK, Activ

Related Disease
Psoriasis

Product Property

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Applications

Application Notes
The IL17A antibody has been reported in applications of ELISA, FC, IP, FuncS, IF, Neut, WB, Inhib, PK, Activ.

Target

Alternative Names
bimekizumab;1418205-77-2;IL17A;interleukin 17A;CTLA8, IL17, interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8);interleukin-17A;cytotoxic T lymphocyte associated protein 8;IL 17;IL 17A;CTLA-8;cytotoxic T-lymphocyte-associated antigen 8;cy

Gene ID
3605

UniProt ID
Q16552

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(Creative Biolabs Cat# TAB-H08, RRID: AB_3112023)

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Biosimilar Overview

Please refer to Bimekizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Bimekizumab.

Related Diseases

Inflammatory Bowel Diseases

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-H08.pdf

MSDS

MSDS - TAB-H08.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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